An Enzyme-Linked Immunosorbent Assay Using Nuclear Antigen for Detection of Feline Herpesvirus 1 Antibody
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چکیده
منابع مشابه
Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
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Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...
متن کاملENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملAn antigen-capture enzyme-linked immunosorbent assay
An antigen-capture enzyme-linked immunosorbent assay (ELISA) was developed to detect and measure isometamidium chloride in the plasma of Oncorhynchus tshawytscha and 0. mykiss. Isometamidium-ovalbunlin conjugate and anti-isometamidium antibodies were used to coat polystyrene plates. The peroxidase saturation technique was used to optirnize the coating antigen concentration; it demonstrated low ...
متن کاملstandardization of an enzyme-linked immunosorbent assay for detection of infectious bronchitis virus antibody.
an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 1999
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063879901100407